The text is styled as the correspondence between the presbyter Pastor and Timotheus. First there is a letter of Pastor (c. 1-6, BHL 6988-6989) telling of a pious man Pudens and his two daughters Pudentiana and Praxedis who lived as virgins in the church founded by their father in their house. The church was dedicated to the name of the presbyter Pastor:
1. [...] Hic itaque a B. Pio doctus, qui et te docuit, domum suam post mortem uxoris suae ecclesiam Christi consecrari cupiens, per nos peccatores ad effectum perduxit: ubi et titulum nomini nostri constituit in urbe Romana, in loco scilicet qui appellatur Vicus Patricii. De hoc ergo Pudente notum tibi facio quia migravit ad omnium Dominum, et reliquit praedictas filias, castitate fultas, omnique lege divina eruditas.
The virgins sell their property and later build the baptistery near the church (c. 2) and many people are baptised there. Emperor Antoninus issues restrictive measures against the Christians. Sixteen years later Pudentiana dies and is buried near her father in the cemetery of Priscilla, while Praxedis continued to live by the mentioned church. She is frequently visited by the presbyter Timotheus and his brother Novatus. Novatus, however, fells ill and dies leaving all his possessions to Pastor and Praxedis. They ask Timotheus in a letter what they should do with them. Then follows the response of Timotheus who allows Praxedis to keep his brother's property (c. 7). Then follows the narrative by Pastor that tells of the dedication of the titulus in the former baths of Novatus (the titulus of Praxedis in the vicus Lateranus, in the text erroneously called the titulus Pudentianae). Two years later during the persecution of Christians by the emperor Antoninus, Praxedis hides the believers in the church but they are denounced:
8. [...] Misitque statim et tenuit multos ex illis: inter quos tenuit Simetrium presbyterum cum aliis viginti duobus: et eos sine audientia gladio puniri praecepit. Quorum corpora noctu beata Praxedis collegit, et in coemeterio Priscillae sub die VII Kalendas Junii sepelivit. Deinde Virgo Domini, nimia corporis afflictione constricta, cum intimis suspiriis ad Dominum ingemuit, et ut ex hoc mundo tutius auferri mereretur oravit. Cujus orationes et lacrymae pervenerunt ad coelum. Nam post dies quinquaginta quinque passionis praedictorum sanctorum, die videlicet duodecimo Kalendas Augusti, migravit ad Dominum. Cujus corpus ego Pastor presbyter sepelivi juxta patrem Pudentem, via Salaria, in coemeterio Priscillae. [...]
(Acta Sanctorum Maii IV, 299-300)